We recently run into such a case and finally found out that variations in the 3D reconstructions were systematically correlated with the grid batches that were used. Sample preparation on cryo-EM grids can give various results, from very thin ice and homogeneous particle distribution (ideal case) to unwanted behavior such as particles around the “holes” or complexes that do not entirely correspond to the one in solution (real life). 4Laboratoire de Physique de la Matière Condensée, Ecole Polytechnique, CNRS, IP Paris, Palaiseau, France.3Laboratoire de Physique des Interfaces et Couches Minces (LPICM), CNRS-UMR 7647, Ecole Polytechnique, IP Paris, Palaiseau, France.2Thales Research and Technology, Palaiseau, France.1Laboratoire de Biologie Structurale de la Cellule, BIOC, Ecole Polytechnique, CNRS-UMR7654, IP Paris, Palaiseau, France.Ramy Kazan 1 Gabrielle Bourgeois 1 Dominique Carisetti 2 Ileana Florea 3 Eric Larquet 4 Jean-Luc Maurice 3 Yves Mechulam 1 François Ozanam 4 Emmanuelle Schmitt 1 Pierre-Damien Coureux 1*
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